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RT-PCR

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When a person is infected, the most common symptoms include fever, cough, and shortness of breath. HOW RT-PCR IS DONE? The samples can be collected by a nasopharyngeal swab or an oropharyngeal swab. For Nasopharyngeal specimen the swab is inserted in the nostril and gently moved forward into the nasopharynx then it is rotated for a specified period time to collect secretions that contain the virus. Once the swabbing is applied, the swab is placed immediately into sterile tube containing a viral transport medium.  The standard method of coronavirus testing is polymerase chain reaction, PCR Which is a method that used widely in molecular biology to make millions to billions of copies of a specific DNA fragment rapidly.  Coronaviruses contain an extraordinarily long single-stranded RNA genome. To detect these viruses with PCR, RNA molecules must be converted into their complementary DNA sequences by reverse transcriptase. Then the newly synthesized DNA can be amplified by st...

Is corona virus human made?

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Corona virus Disease caused by SARS-CoV2 is originated from china. On 31st december,2019 china reported cluster of cases in Wuhan by unknown pathogen to WHO. American president Donald trump and many other representatives claimed that it was created in Wuhan center for disease control or Wuhan institute of virology. This news become more tricky when china did not allow WHO for investigation in laboratory. So what is the truth behind all this. Is really coronavirus human made? One article published in The Lancet journal shows that initially 27 cases out of 41 was linked to Wuhan wholesale seafood market. One way to find is coronavirus human made or naturally originated is comparing genome sequences. By decoding the genome sequence one can identify organoleptic characters in human like hair colour,eye colour and sex of human.So it can act similarly in case of virus. So decoding genome of virus is the best way to know its origin. Gene is made up of nucleotides. There are almost billion nuc...