RT-PCR
When a person is infected, the most common symptoms include fever, cough, and shortness of breath. HOW RT-PCR IS DONE? The samples can be collected by a nasopharyngeal swab or an oropharyngeal swab. For Nasopharyngeal specimen the swab is inserted in the nostril and gently moved forward into the nasopharynx then it is rotated for a specified period time to collect secretions that contain the virus. Once the swabbing is applied, the swab is placed immediately into sterile tube containing a viral transport medium. The standard method of coronavirus testing is polymerase chain reaction, PCR Which is a method that used widely in molecular biology to make millions to billions of copies of a specific DNA fragment rapidly. Coronaviruses contain an extraordinarily long single-stranded RNA genome. To detect these viruses with PCR, RNA molecules must be converted into their complementary DNA sequences by reverse transcriptase. Then the newly synthesized DNA can be amplified by st...